human recombinant spp1 Search Results


spp1 recombinant human protein  (Thermo Fisher)
N/A
Manufactured by Sino Biological (China); Distributed by Life Technologies. Secreted Phosphoprotein 1 (SPP1) recombinant human protein is supplied as a lyophilized powder. This protein is suitable for use in analysis of protein structure. In general,
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opnb  (OriGene)
90
OriGene opnb
Fig. 3 OPN isoforms differentially influence macrophage accumula- tion in vivo. a To determine if OPN isoforms differentially promote macrophage accumulation, histology sections from WT, OPN−/−, and OPN isoform a-, b-, or c-treated animals were stained for CD68 (macrophage marker; pink stain) 5 days post HLI. The 20× repre- sentative images show detail. The 2.5× images stitched together across the hindlimb demonstrate tissue-wide differences. Scale bars for 2.5× stitched = 200 μm. Scale bars for 20× = 50 μm. b To quantitatively assess macrophage accumulation in response to OPN isoforms, qRT- PCR on mRNA isolated from the muscles of the IL was used to quantify CD68 expression. CD68 expression was compared across all treatment groups at day 7; #p < 0.05 vs. OPN−/−, LV-GFP; *p < 0.05 vs. OPN−/−, LV-GFP, and <t>LV-OPNb,</t> †p < 0.001 vs. OPN−/−, LV- <t>GFP,</t> <t>LV-OPNa,</t> and LV-OPNb; n = 4
Opnb, supplied by OriGene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+recombinant+spp1/Osteopontin+(SPP1)+(NM_001040058)+Human+Recombinant+Protein/pm29959420-92-21-29
Average 90 stars, based on 1 article reviews
opnb - by Bioz Stars, 2026-10
90/100 stars
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recombinant opn  (Boster Bio)
91
Boster Bio recombinant opn
Fig. 3 OPN isoforms differentially influence macrophage accumula- tion in vivo. a To determine if OPN isoforms differentially promote macrophage accumulation, histology sections from WT, OPN−/−, and OPN isoform a-, b-, or c-treated animals were stained for CD68 (macrophage marker; pink stain) 5 days post HLI. The 20× repre- sentative images show detail. The 2.5× images stitched together across the hindlimb demonstrate tissue-wide differences. Scale bars for 2.5× stitched = 200 μm. Scale bars for 20× = 50 μm. b To quantitatively assess macrophage accumulation in response to OPN isoforms, qRT- PCR on mRNA isolated from the muscles of the IL was used to quantify CD68 expression. CD68 expression was compared across all treatment groups at day 7; #p < 0.05 vs. OPN−/−, LV-GFP; *p < 0.05 vs. OPN−/−, LV-GFP, and <t>LV-OPNb,</t> †p < 0.001 vs. OPN−/−, LV- <t>GFP,</t> <t>LV-OPNa,</t> and LV-OPNb; n = 4
Recombinant Opn, supplied by Boster Bio, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+recombinant+spp1/SPP1+Human%2C+Osteopontin+Human+Recombinant+Protein%2C+HEK/pmc08936047-293-9-11
Average 91 stars, based on 1 article reviews
recombinant opn - by Bioz Stars, 2026-10
91/100 stars
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osteopontin  (OriGene)
90
OriGene osteopontin
Fig. 3 OPN isoforms differentially influence macrophage accumula- tion in vivo. a To determine if OPN isoforms differentially promote macrophage accumulation, histology sections from WT, OPN−/−, and OPN isoform a-, b-, or c-treated animals were stained for CD68 (macrophage marker; pink stain) 5 days post HLI. The 20× repre- sentative images show detail. The 2.5× images stitched together across the hindlimb demonstrate tissue-wide differences. Scale bars for 2.5× stitched = 200 μm. Scale bars for 20× = 50 μm. b To quantitatively assess macrophage accumulation in response to OPN isoforms, qRT- PCR on mRNA isolated from the muscles of the IL was used to quantify CD68 expression. CD68 expression was compared across all treatment groups at day 7; #p < 0.05 vs. OPN−/−, LV-GFP; *p < 0.05 vs. OPN−/−, LV-GFP, and <t>LV-OPNb,</t> †p < 0.001 vs. OPN−/−, LV- <t>GFP,</t> <t>LV-OPNa,</t> and LV-OPNb; n = 4
Osteopontin, supplied by OriGene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+recombinant+spp1/Osteopontin+(SPP1)+(NM_000582)+Human+Recombinant+Protein/pm23794266-81-10-30
Average 90 stars, based on 1 article reviews
osteopontin - by Bioz Stars, 2026-10
90/100 stars
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recombinant human osteopontin (opn) protein  (Bio-Techne corporation)
95
Bio-Techne corporation recombinant human osteopontin (opn) protein
Fig. 3 OPN isoforms differentially influence macrophage accumula- tion in vivo. a To determine if OPN isoforms differentially promote macrophage accumulation, histology sections from WT, OPN−/−, and OPN isoform a-, b-, or c-treated animals were stained for CD68 (macrophage marker; pink stain) 5 days post HLI. The 20× repre- sentative images show detail. The 2.5× images stitched together across the hindlimb demonstrate tissue-wide differences. Scale bars for 2.5× stitched = 200 μm. Scale bars for 20× = 50 μm. b To quantitatively assess macrophage accumulation in response to OPN isoforms, qRT- PCR on mRNA isolated from the muscles of the IL was used to quantify CD68 expression. CD68 expression was compared across all treatment groups at day 7; #p < 0.05 vs. OPN−/−, LV-GFP; *p < 0.05 vs. OPN−/−, LV-GFP, and <t>LV-OPNb,</t> †p < 0.001 vs. OPN−/−, LV- <t>GFP,</t> <t>LV-OPNa,</t> and LV-OPNb; n = 4
Recombinant Human Osteopontin (Opn) Protein, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+recombinant+spp1/Recombinant+Human+Osteopontin+(OPN)+Protein/bio-techne+corporation___1433-op
Average 95 stars, based on 1 article reviews
recombinant human osteopontin (opn) protein - by Bioz Stars, 2026-10
95/100 stars
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spp1 human, hek - recombinant human osteopontin, hek  (Aviva Systems)
N/A
Product Introduction: Osteopontin is a glycoprotein that was first identified in osteoblasts and is involved in bone remodeling, immune functions in fibroblasts, macrophages, and lymphocytes during inflammation and wound healing. SPP1 binds tightly to hydroxyapatite.
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recombinant human osteopontin(spp1),partial  (Cusabio)
N/A
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osteopontin spp1 nm 001040058 human recombinant protein  (OriGene)
N/A
Purified protein of Homo sapiens secreted phosphoprotein 1 SPP1 transcript variant 1 residues 17 314aa secretory expressed with GP67 signal peptide with C terminal DDK tag expressed in sf9 20ug
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recombinant human osteopontin/opn/spp-1/spp1  (Beijing Solarbio Science)
N/A
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spp1 human - recombinant human osteopontin  (Aviva Systems)
N/A
SPP1; Recombinant Human Osteopontin; Recombinant Human Osteopontin
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osteopontin spp1 nm 000582 human recombinant protein  (OriGene)
N/A
Recombinant protein of human secreted phosphoprotein 1 SPP1 transcript variant 2
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osteopontin spp1 nm 001040060 human recombinant protein  (OriGene)
N/A
Purified recombinant protein of human secreted phosphoprotein 1 SPP1 transcript variant 3 with C terminal MYC DDK tag secretory expressed in HEK293 cells 20ug
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Image Search Results


Fig. 3 OPN isoforms differentially influence macrophage accumula- tion in vivo. a To determine if OPN isoforms differentially promote macrophage accumulation, histology sections from WT, OPN−/−, and OPN isoform a-, b-, or c-treated animals were stained for CD68 (macrophage marker; pink stain) 5 days post HLI. The 20× repre- sentative images show detail. The 2.5× images stitched together across the hindlimb demonstrate tissue-wide differences. Scale bars for 2.5× stitched = 200 μm. Scale bars for 20× = 50 μm. b To quantitatively assess macrophage accumulation in response to OPN isoforms, qRT- PCR on mRNA isolated from the muscles of the IL was used to quantify CD68 expression. CD68 expression was compared across all treatment groups at day 7; #p < 0.05 vs. OPN−/−, LV-GFP; *p < 0.05 vs. OPN−/−, LV-GFP, and LV-OPNb, †p < 0.001 vs. OPN−/−, LV- GFP, LV-OPNa, and LV-OPNb; n = 4

Journal: Laboratory investigation; a journal of technical methods and pathology

Article Title: Osteopontin isoforms differentially promote arteriogenesis in response to ischemia via macrophage accumulation and survival.

doi: 10.1038/s41374-018-0094-8

Figure Lengend Snippet: Fig. 3 OPN isoforms differentially influence macrophage accumula- tion in vivo. a To determine if OPN isoforms differentially promote macrophage accumulation, histology sections from WT, OPN−/−, and OPN isoform a-, b-, or c-treated animals were stained for CD68 (macrophage marker; pink stain) 5 days post HLI. The 20× repre- sentative images show detail. The 2.5× images stitched together across the hindlimb demonstrate tissue-wide differences. Scale bars for 2.5× stitched = 200 μm. Scale bars for 20× = 50 μm. b To quantitatively assess macrophage accumulation in response to OPN isoforms, qRT- PCR on mRNA isolated from the muscles of the IL was used to quantify CD68 expression. CD68 expression was compared across all treatment groups at day 7; #p < 0.05 vs. OPN−/−, LV-GFP; *p < 0.05 vs. OPN−/−, LV-GFP, and LV-OPNb, †p < 0.001 vs. OPN−/−, LV- GFP, LV-OPNa, and LV-OPNb; n = 4

Article Snippet: For macrophage polarization studies, 3 h after plating cells were stimulated with 10% FBS–RPMI with 100 ng/mL purified recombinant human OPNa, OPNb, or OPNc (cat# TP304803, TP305118, and TP31059; Origene, Rockville, MD), or were stimulated with 20 ng/mL of either interferon gamma (INFγ) or interleukin (IL)-4 for 48 h (cat# 554587 and 550067; BD Biosciences) as positive controls for macrophage polarization.

Techniques: In Vivo, Staining, Marker, Quantitative RT-PCR, Isolation, Muscles, Expressing

Fig. 6 Effects of MCP-1 and OPN isoforms on macrophage migration in vitro. Peritoneal macrophages were used as an in vitro cell model to measure isoform effects on macrophage migration using a modified Boyden chamber assay. a Monocyte chemoattractant protein-1 (MCP-1), a known stimulus for macrophage migration, was used to first determine if OPN−/−and WT macrophages exhibit differences in MCP-1-mediated migration after 2 h (n = 4, *p = 0.0027 vs. WT−no trx, †p < 0.001 vs. WT+ MCP-1). b To assess if OPN isoforms differentially promote macrophage migration, OPN−/−macrophages were stimulated with 50 ng/mL of recombinant OPN a, b, or c and macrophage migration was quantified after 2 h (n = 4, *p < 0.05 vs. no trx and OPNb, †p < 0.001 vs. no trx, OPNa, and OPNb). c Proposed mechanism by which OPN isoforms a and c increase arteriogenesis in vivo by increasing macrophage migration and survival

Journal: Laboratory investigation; a journal of technical methods and pathology

Article Title: Osteopontin isoforms differentially promote arteriogenesis in response to ischemia via macrophage accumulation and survival.

doi: 10.1038/s41374-018-0094-8

Figure Lengend Snippet: Fig. 6 Effects of MCP-1 and OPN isoforms on macrophage migration in vitro. Peritoneal macrophages were used as an in vitro cell model to measure isoform effects on macrophage migration using a modified Boyden chamber assay. a Monocyte chemoattractant protein-1 (MCP-1), a known stimulus for macrophage migration, was used to first determine if OPN−/−and WT macrophages exhibit differences in MCP-1-mediated migration after 2 h (n = 4, *p = 0.0027 vs. WT−no trx, †p < 0.001 vs. WT+ MCP-1). b To assess if OPN isoforms differentially promote macrophage migration, OPN−/−macrophages were stimulated with 50 ng/mL of recombinant OPN a, b, or c and macrophage migration was quantified after 2 h (n = 4, *p < 0.05 vs. no trx and OPNb, †p < 0.001 vs. no trx, OPNa, and OPNb). c Proposed mechanism by which OPN isoforms a and c increase arteriogenesis in vivo by increasing macrophage migration and survival

Article Snippet: For macrophage polarization studies, 3 h after plating cells were stimulated with 10% FBS–RPMI with 100 ng/mL purified recombinant human OPNa, OPNb, or OPNc (cat# TP304803, TP305118, and TP31059; Origene, Rockville, MD), or were stimulated with 20 ng/mL of either interferon gamma (INFγ) or interleukin (IL)-4 for 48 h (cat# 554587 and 550067; BD Biosciences) as positive controls for macrophage polarization.

Techniques: Migration, In Vitro, Boyden Chamber Assay, Recombinant, In Vivo